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. 1996 Sep 1;318(Pt 2):597–602. doi: 10.1042/bj3180597

Hepatic regeneration induces changes in lipoprotein lipase activity in several tissues and its re-expression in the liver.

R Sabugal 1, M Q Robert 1, J Julve 1, J Auwerx 1, M Llobera 1, J Peinado-Onsurbe 1
PMCID: PMC1217662  PMID: 8809052

Abstract

We examined the expression of lipoprotein lipase (LPL) gene and LPL activity following a two-thirds hepatectomy and during liver regeneration. In most of the tissues studied, LPL activity increased a few hours after partial hepatectomy, but soon returned to normal levels. The greatest increase was found in the adrenal glands, plasma and liver. This increase in LPL activity in the liver could be partially due to an increase in the influx of the enzyme from extrahepatic tissues. There is, however, also a re-expression of LPL mRNA in the liver after partial hepatectomy (during the first hours). It is well known that LPL is expressed in the liver of neonatal animals, but progressively decreases during post-natal development, to reach adult levels around the time of weaning. Our results show by the first time that the remaining liver re-expresses LPL gene during the regeneration process and that the hepatocytes de-differentiate and acquire some of the neonatal characteristics. The increase in LPL mRNA will contribute to the rise in LPL activity after hepatectomy. This presence of LPL could enable the liver to take up fatty acids from the circulating triacylglycerols, which are needed as energetic and plastic substrates during the process of hepatic regeneration.

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Selected References

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  1. Ashby P., Tolson A. M., Robinson D. S. The heterogeneity of the lipoprotein lipase of rat epididymal adipose tissue. Biochem J. 1978 May 1;171(2):305–311. doi: 10.1042/bj1710305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball K. L., Speake B. K., Robinson D. S. Effects of adrenaline on the turnover of lipoprotein lipase in rat adipose tissue. Biochim Biophys Acta. 1986 Jul 18;877(3):399–405. doi: 10.1016/0005-2760(86)90205-5. [DOI] [PubMed] [Google Scholar]
  3. Burgaya F., Peinado J., Vilaró S., Llobera M., Ramírez I. Lipoprotein lipase activity in neonatal-rat liver cell types. Biochem J. 1989 Apr 1;259(1):159–166. doi: 10.1042/bj2590159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chajek-Shaul T., Friedman G., Stein O., Etienne J., Stein Y. Endogenous plasma lipoprotein lipase activity in fed and fasting rats may reflect the functional pool of endothelial lipoprotein lipase. Biochim Biophys Acta. 1985 Dec 4;837(3):271–278. doi: 10.1016/0005-2760(85)90050-5. [DOI] [PubMed] [Google Scholar]
  5. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  6. Cruise J. L., Knechtle S. J., Bollinger R. R., Kuhn C., Michalopoulos G. Alpha 1-adrenergic effects and liver regeneration. Hepatology. 1987 Nov-Dec;7(6):1189–1194. doi: 10.1002/hep.1840070604. [DOI] [PubMed] [Google Scholar]
  7. Curtin N. J., Snell K. Enzymic retrodifferentiation during hepatocarcinogenesis and liver regeneration in rats in vivo. Br J Cancer. 1983 Oct;48(4):495–505. doi: 10.1038/bjc.1983.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. De Gasquet P., Griglio S., Pequignot-Planche E., Malewiak M. I. Diurnal changes in plasma and liver lipids and lipoprotein lipase activity in heart and adipose tissue in rats fed a high and low fat diet. J Nutr. 1977 Feb;107(2):199–212. doi: 10.1093/jn/107.2.199. [DOI] [PubMed] [Google Scholar]
  9. Enerbäck S., Gimble J. M. Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-transcriptional level. Biochim Biophys Acta. 1993 Aug 11;1169(2):107–125. doi: 10.1016/0005-2760(93)90196-g. [DOI] [PubMed] [Google Scholar]
  10. Enerbäck S., Semb H., Tavernier J., Bjursell G., Olivecrona T. Tissue-specific regulation of guinea pig lipoprotein lipase; effects of nutritional state and of tumor necrosis factor on mRNA levels in adipose tissue, heart and liver. Gene. 1988 Apr 15;64(1):97–106. doi: 10.1016/0378-1119(88)90484-2. [DOI] [PubMed] [Google Scholar]
  11. Giralt M., Martin I., Vilaró S., Villarroya F., Mampel T., Iglesias R., Viñas O. Lipoprotein lipase mRNA expression in brown adipose tissue: translational and/or posttranslational events are involved in the modulation of enzyme activity. Biochim Biophys Acta. 1990 Apr 6;1048(2-3):270–273. doi: 10.1016/0167-4781(90)90066-b. [DOI] [PubMed] [Google Scholar]
  12. Gove C. D., Hems D. A. Fatty acid synthesis in the regenerating liver of the rat. Biochem J. 1978 Jan 15;170(1):1–8. doi: 10.1042/bj1700001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gåfvels M., Vilaró S., Olivecrona T. Lipoprotein lipase in guinea-pig adrenals: activity, mRNA, immunolocalization and regulation by ACTH. J Endocrinol. 1991 May;129(2):213–220. doi: 10.1677/joe.0.1290213. [DOI] [PubMed] [Google Scholar]
  14. Haber B. A., Mohn K. L., Diamond R. H., Taub R. Induction patterns of 70 genes during nine days after hepatectomy define the temporal course of liver regeneration. J Clin Invest. 1993 Apr;91(4):1319–1326. doi: 10.1172/JCI116332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Holness M. J., Schofield P. S., Sugden M. C. Altered interactions between glycogenesis and lipid synthesis after liver resection: specific effects of liver regeneration, coupled with non-specific effects of surgery. Clin Sci (Lond) 1989 Mar;76(3):317–322. doi: 10.1042/cs0760317. [DOI] [PubMed] [Google Scholar]
  16. Jansen H., De Greef W. J. Heparin-releasable lipase activity of rat adrenals, ovaries and testes. Biochem J. 1981 Jun 15;196(3):739–745. doi: 10.1042/bj1960739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Koppel J., Loyer P., Maucuer A., Rehák P., Manceau V., Guguen-Guillouzo C., Sobel A. Induction of stathmin expression during liver regeneration. FEBS Lett. 1993 Sep 27;331(1-2):65–70. doi: 10.1016/0014-5793(93)80298-9. [DOI] [PubMed] [Google Scholar]
  18. Kotlar T. J., Borensztajn J. Oscillatory changes in muscle lipoprotein lipase activity of fed and starved rats. Am J Physiol. 1977 Oct;233(4):E316–E319. doi: 10.1152/ajpendo.1977.233.4.E316. [DOI] [PubMed] [Google Scholar]
  19. Leffert H. L., Koch K. S., Moran T., Rubalcava B. Hormonal control of rat liver regeneration. Gastroenterology. 1979 Jun;76(6):1470–1482. [PubMed] [Google Scholar]
  20. Michalopoulos G. K. Liver regeneration: molecular mechanisms of growth control. FASEB J. 1990 Feb 1;4(2):176–187. [PubMed] [Google Scholar]
  21. Michalopoulos G. Liver regeneration and growth factors: old puzzles and new perspectives. Lab Invest. 1992 Oct;67(4):413–415. [PubMed] [Google Scholar]
  22. Miller W. C., Gorski J., Oscai L. B., Palmer W. K. Epinephrine-activation of heparin-nonreleasable lipoprotein lipase in 3 skeletal muscle fiber types of the rat. Biochem Biophys Res Commun. 1989 Oct 31;164(2):615–619. doi: 10.1016/0006-291x(89)91504-0. [DOI] [PubMed] [Google Scholar]
  23. Mitchell J. R., Jacobsson A., Kirchgessner T. G., Schotz M. C., Cannon B., Nedergaard J. Regulation of expression of the lipoprotein lipase gene in brown adipose tissue. Am J Physiol. 1992 Sep;263(3 Pt 1):E500–E506. doi: 10.1152/ajpendo.1992.263.3.E500. [DOI] [PubMed] [Google Scholar]
  24. Nestel P. J., Austin W. Relationship between adipose lipoprotein lipase activity and compounds which affect intracellular lipolysis. Life Sci. 1969 Feb 15;8(4):157–164. doi: 10.1016/0024-3205(69)90075-7. [DOI] [PubMed] [Google Scholar]
  25. Noda C., Ichihara A. Regulation of liver-specific gene expression. Cell Struct Funct. 1993 Aug;18(4):189–194. doi: 10.1247/csf.18.189. [DOI] [PubMed] [Google Scholar]
  26. Peinado-Onsurbe J., Soler C., Soley M., Llobera M., Ramírez I. Lipoprotein lipase and hepatic lipase activities are differentially regulated in isolated hepatocytes from neonatal rats. Biochim Biophys Acta. 1992 Apr 8;1125(1):82–89. doi: 10.1016/0005-2760(92)90159-s. [DOI] [PubMed] [Google Scholar]
  27. Peinado-Onsurbe J., Staels B., Deeb S., Ramirez I., Llobera M., Auwerx J. Neonatal extinction of liver lipoprotein lipase expression. Biochim Biophys Acta. 1992 Jul 15;1131(3):281–286. doi: 10.1016/0167-4781(92)90026-v. [DOI] [PubMed] [Google Scholar]
  28. Ramírez I., Kryski A. J., Ben-Zeev O., Schotz M. C., Severson D. L. Characterization of triacylglycerol hydrolase activities in isolated myocardial cells from rat heart. Biochem J. 1985 Nov 15;232(1):229–236. doi: 10.1042/bj2320229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Staels B., Auwerx J. Perturbation of developmental gene expression in rat liver by fibric acid derivatives: lipoprotein lipase and alpha-fetoprotein as models. Development. 1992 Aug;115(4):1035–1043. doi: 10.1242/dev.115.4.1035. [DOI] [PubMed] [Google Scholar]
  30. Trentalance A., Bruscalupi G., Conti Devirgiliis L., Leoni S., Mangiantini M. T., Rossini L., Spagnuolo S., Erickson S. K. Changes in lipoprotein binding and uptake by hepatocytes during rat liver regeneration. Biosci Rep. 1989 Apr;9(2):231–241. doi: 10.1007/BF01116000. [DOI] [PubMed] [Google Scholar]
  31. Uriel J. Retrodifferentiation and the fetal patterns of gene expression in cancer. Adv Cancer Res. 1979;29:127–174. doi: 10.1016/s0065-230x(08)60847-7. [DOI] [PubMed] [Google Scholar]
  32. Vilaró S., Llobera M., Bengtsson-Olivecrona G., Olivecrona T. Synthesis of lipoprotein lipase in the liver of newborn rats and localization of the enzyme by immunofluorescence. Biochem J. 1988 Jan 15;249(2):549–556. doi: 10.1042/bj2490549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Vilaró S., Llobera M. Uptake and metabolism of Intralipid by rat liver: an electron-microscopic study. J Nutr. 1988 Aug;118(8):932–940. doi: 10.1093/jn/118.8.932. [DOI] [PubMed] [Google Scholar]
  34. Wewer U. M., Engvall E., Paulsson M., Yamada Y., Albrechtsen R. Laminin A, B1, B2, S and M subunits in the postnatal rat liver development and after partial hepatectomy. Lab Invest. 1992 Mar;66(3):378–389. [PubMed] [Google Scholar]
  35. Wing D. R., Robinson D. S. Clearing-factor lipase in adipose tissue. Studies with puromycin and actinomycin. Biochem J. 1968 Feb;106(3):667–676. doi: 10.1042/bj1060667. [DOI] [PMC free article] [PubMed] [Google Scholar]

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