Fig. 2.
Investigation of uptake efficiency and antitumor immune response effect of DEXXCL1 in vitro. A Flow cytometric analysis of DEX and DEXXCL1 uptake in DC cells derived from lymph node of C57BL/6 mice 24 h after incubation. NC (negative control) refers to PBS treated DCs (n = 3). B Flow cytometry for analyzing levels of surface proteins (MHCI and CCR7) in DCs treated with PBS, DEX and DEXXCL1 (n = 3). C Measurement of IL12p70 cytokine levels in the culture supernatants were quantitated by cytokine array (n = 3). D Representative flow cytometric analysis of CD69+CD8+ for 12 h incubation or IFN-γ+ CD8+ for 72-h incubation in CD8+splenic T cells harvested from immunized C57BL/6mice with DEXorDEXxcl1 activated DCs (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001)