Fig. 5. Transmission of TMUV SD in chickens via mosquito bites. (a) Schematic representation of the infection of chickens via mosquito bites. (b) The occurrence of viremia in chickens bitten by infected mosquitoes was detected by virus titration with the blood samples collected at 2, 3 and 4 days post-bites on BHK cells. (c) TMUV-specific antibody test: the TMUV-specific antibodies in the serum samples of three inoculated chickens, five bitten chickens, and three contact chickens were measured by ELISA after 2 weeks post-bites. A positive seroconversion is noted when the PI value exceeds 18.4%.
