Table 2.
Particle volume analysis for cycA and cycB mRNAs in the knockdown backgrounds
| Measurements | Cup-YFP Stages 2-4 |
Bru1-GFP Stages 2-4 |
Bru1-GFP Stages 5-7 |
|||
|---|---|---|---|---|---|---|
| control | bru1RNAi | control | cupRNAi | control | cupRNAi | |
| cycA mRNA | ||||||
| mRNA particle inside condensate (%) | 45% | 14% | 45% | 23% | 51% | 29% |
| mRNA particle outside condensate (%) | 55% | 86% | 55% | 77% | 49% | 71% |
| Average volume of mRNA inside condensates (μm3) | 0.0039 | 0.0011 | 0.0038 | 0.0034 | 0.0035 | 0.0033 |
| Average volume of mRNA outside condensates (μm3) | 0.0014 | 0.0004 | 0.0015 | 0.0018 | 0.0014 | 0.0020 |
| Fold increase of mRNA volume inside vs. outside condensate | 2.8 | 2.6 | 2.5 | 1.9 | 2.5 | 1.7 |
| cycB mRNA | ||||||
| mRNA particle inside condensate (%) | 41% | 21% | 43% | 25% | 48% | 27% |
| mRNA particle outside condensate (%) | 59% | 79% | 57% | 75% | 52% | 73% |
| Average volume of mRNA inside condensates (μm3) | 0.0038 | 0.0033 | 0.0038 | 0.0036 | 0.0041 | 0.0041 |
| Average volume of mRNA outside condensates (μm3) | 0.0013 | 0.0015 | 0.0012 | 0.0018 | 0.0016 | 0.0022 |
| Fold increase of mRNA volume inside vs. outside condensate | 3.0 | 2.3 | 3.1 | 2.0 | 2.5 | 1.9 |