Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2025 Apr 18;56(7):1872–1882. doi: 10.1161/STROKEAHA.124.048964

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2025 The Authors.

Stroke is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

PMC Copyright notice

Figure 4. — Hypoxic-preconditioned mesenchymal stem cells (HP-MSCs) enhance differentiation of neural stem cells (NSCs) into more complex neurons than normoxic-preconditioned MSCs (NP-MSCs). A, Overview of experimental design. B, Representative fluorescent images (10×) of βIIIT (βIII-tubulin)⁺ cells (differentiated NSCs) in co-culture with NP-MSCs or HP-MSCs (+) or empty inserts (−). C, Quantification of βIIIT⁺ area normalized to the cell numbers (4',6-diamidino-2-phenylindole [DAPI] count) relative to the NP-MSCs (−) condition. D, Quantification of neurite length of βIIIT⁺ neurons. E, Number of intersections of βIIIT⁺ neurons with circles of increasing radius in Sholl analysis. F, Quantification of Sholl analysis by area under the curve. Data represent mean±SD. All: n=13–15 per condition out of 3 independent experiments. A.U. indicates arbitrary unit; bFGF, basic fibroblast growth factor; and EGF, epidermal growth factor. *P<0.05, ***P<0.001, and ****P<0.0001.