Table 7.
Oligonucleotide primers sequence, accession number, annealing temperature and PCR product size of immune and antioxidant genes used in real time PCR.
| Gene | Primer | Product length (bp) | Annealing Temperature (°C) | Accession number | Source |
|---|---|---|---|---|---|
| IFN-γ |
F5′-GATGATCTGCAGATCCAGCGCA-3 R5′-AGAGATTCTGACTTCTCTTCCG-3′ |
106 | 60 | AY575608.1 | Current study |
| IL-4 |
F5′-GGAATTGAGCTTAGGCGTAT-3′ R5′-TCTTGCTTGCCAGGCTGCTGA-3′ |
94 | 62 | AY575607.1 | Current study |
| TNF-α |
F5′-ACTATGCCGAGTCTGGGCAGG-3′ R5′-GCTTGGAGCCCAGCCCTGAG-3′ |
164 | 58 | AY513771.1 | Current study |
| MYD88 |
F5′-CTGAAGCAGCAGCAGGAGGCAT-3′ R5′-GTCGCGAATAGTGATGCCTGCCA-3′ |
102 | 58 | NM_001166183.1 | Current study |
| CCL5 |
F5′-′CACCAGCAGCAAGTGCTCCATG-3′ R5′-CACTTCTTCTCTGGGTTGGCGCA-3′ |
84 | 60 | XM_005693201.3 | Current study |
| TLR4 |
F5′-TAGGAAGTCTACAAGCCCTTCT-3′ R5′-CAGGAAACTTATCAAAGTCACA-3′ |
81 | 62 | MW201968.1 | Current study |
| TLR9 |
F5′-AGCAGGAGATGTTTACCCGCCTC-3′ R5′-AGCACTCGCAGGCCGGTCAGCG-3′ |
115 | 64 | HQ717159.1 | Current study |
| LTF |
F5′-GTGGTGTCTCGGAGCGATAG-3′ R5′-CATACGTTGGTCTGCCTCCA-3′ |
184 | 62 | AY792499.1 | Current study |
| PRLR |
F5′-CATGGCATGGCCACTGCTCCA-3′ R5′-CTTCGAGTACTCCTTGCTGGTT-3′ |
160 | 60 | FJ901299.1 | Current study |
| CAT |
F5′-CAGTAGGAGACAAACTCAATG-3′ R5′-ACGACTCTCTCAGGAATTCTC-3′ |
121 | 62 | GQ204786.1 | Current study |
| GPX1 |
F5′-CGAGGAGATCCTGAATTGCCTGA-3′ R5′-ACCTCGCACTTTTCGAAGAGC-3′ |
54 | 60 | JF728302.1 | Current study |
| KEAP1 |
F5′-TGAGAGTATCGGAGGCTACGCA-3′ R5′-CGCTAGGCCTGGGTTCCGGCT-3′ |
95 | 62 | XM_027969637.2 | Current study |
| OXSR1 |
F5′-ACAGATTCACAGAGATGTGAAAG-3′ R5′-CCACGAACCTGTTCCATAACT-3′ |
186 | 58 | XM_027958018.2 | Current study |
| ATOX1 |
F5′-GCAGCCACCACCTCCTCCTCAA-3′ R5′-GTGCTCAGAGTTGATGCAGAC-3′ |
122 | 58 | XM_005683194.3 | Current study |
| GST |
F5′-TGGCTGCAGCCGGAGTGGAGTT-3′ R5′-TGGCAACGTAGTTGAGAATGGC-3′ |
162 | 64 | AJ238319.1 | Current study |
| Nrf2 |
F5′-TCACAAGGGAACAGCACTGCAG-3′ R5′-AGCCTCCAAGCGGCTTGAATG-3′ |
129 | 64 | NM_001314327.1 | Current study |
| ß. actin |
F5′-GCAAAGACCTCTACGCCAAC-3′ R5′-TGATCTTCATCGTGCTGGGT-3′ |
114 | 60 | NM_001009784.3 | Current study |
IFN-γ interferon gamma, IL-4 iterleukin-4, TNF-α tumor necrosis factor-alpha, MYD88 myeloid differentiation primary response 88, CCL5 chemokine (C-C motif) ligand 5, TLR4 toll-like receptor 4, TLR9 toll-like receptor 9, LTF lactoferrin, PRLR prolactin receptor, CAT catalase, GPX1 glutathione peroxidase 1, KEAP1 Kelch-like ECH-associated protein 1, OXSR1 oxidative stress responsive kinase 1, ATOX1 antioxidant 1 copper chaperone 1, GST glutathione S transferase, Nrf2 nuclear factor-erythroid factor 2-related factor.