. 2025 Jun 12;31(5):729–738. doi: 10.1007/s12298-025-01603-4

Table 2.

Primer information for candidate reference genes in the roots of bermudagrass under alkaline salt stress

Gene ID	Primer sequence(5′-3′)	Amplification product length (bp)	Amplification efficiency	R²
β-tubulin	GCCGAGAACTGCGACTG	108	85.76	0.986
β-tubulin	CCACATCTGCTGCGTGAG	108	85.76	0.986
Actin	GAATGGTCAAGGCGGGTTT	106	93.56	0.992
Actin	CGTAGTCCAGGGCAATGTAG	106	93.56	0.992
PP2A	ACTACTTCCCCTTGACGGC	183	119.50	0.991
PP2A	AAATACCCCAACCACATCG	183	119.50	0.991
CACS	TCCTGAAGGTTTGGGAGAAG	169	97.72	0.942
CACS	AAATAACAGTTCCGAAGAGCC	169	97.72	0.942
EF1α	TGTCCCTGTCGGTCGTGTT	112	100.80	0.996
EF1α	AGCCTCATGGTGCATCTCA	112	100.80	0.996
TIP41	AATTGACCTTGTCGCTCTTTC	126	89.55	0.994
TIP41	GTCGGCATTACCCTCACTCT	126	89.55	0.994
GAPDH	CCCTTCATCACCACCGACTA	157	117.46	0.995
GAPDH	GAATCTCCTCTGGGTTCCTG	157	117.46	0.995