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. 1997 Aug 15;326(Pt 1):17–20. doi: 10.1042/bj3260017

Rac1 is required for cell proliferation and G2/M progression.

K A Moore 1, R Sethi 1, A M Doanes 1, T M Johnson 1, J B Pracyk 1, M Kirby 1, K Irani 1, P J Goldschmidt-Clermont 1, T Finkel 1
PMCID: PMC1218631  PMID: 9337845

Abstract

We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

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Selected References

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