Figure 2. TEAD1 foci are transcriptionally inactive.

(A–F) Representative immunofluorescence images of TEAD foci (green) and active transcription markers (red): RNAPII2 in UOK121 (A) and UOK342 (B), H3K27ac in UOK121 (C) and UOK342 (D), and UOK121 (E) and UOK342 (F). Insets show magnified boxed regions of merged channels with adjusted intensity. Scale bars = 2 μm. Nuclear boundaries indicated by dashed lines.

(G) Quantitative assessment and statistical evaluation of co-localization of TEAD1 with active transcription markers around foci and control regions. Data are presented as mean ± SEM.

(H-I) Representative images of TEAD1 foci and Fluorescence in Situ Hybridization (FISH) of nascent Cyr61 mRNA in UOK121 (H) and UOK342 (I). Arrows indicating Cyr61 nascent mRNA signals and TEAD1 foci are highlighted with 50×50 pixel squares. Nuclear boundaries are indicated by dotted lines.

(J) Average images centered on TEAD1 foci, showing a lack of active transcription with decreased average Cyr61 intensity (red) in UOK121 (top, n=35) and UOK342 (bottom, n=20). Scale bars are 1μm.

(K) Line plots of TEAD1 foci and Cyr61 average intensity along the dashed line shown in (J) for UOK121 (top) and UOK342 (bottom). Blue line: TEAD1 intensity; magenta line: Cyr61 intensity.

Statistical significance determined by one-way ANOVA: ns, p > 0.05; *, p ≤ 0.05; ****, p ≤ 0.0001.