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[Preprint]. 2025 May 3:2025.05.02.651992. [Version 1] doi: 10.1101/2025.05.02.651992

Figure 3. Co-localization of TEAD1 foci with heterochromatin markers in different RCC cell lines.

Figure 3.

(A-D) Representative immunofluorescence images of TEAD1 (green) and H3K9me3 (red) in UOK121 (A) and UOK342 (C) cells (scale bars are 5μm). Insets show magnified images of boxed regions. Co-localization of TEAD1 foci and H3K9me3 in UOK121(B) and UOK342(D) compared to the more diffused TEAD1 region. Data are presented as scatterplots with mean ± SEM.

(E-H) Representative immunofluorescence images illustrate the co-localization of TEAD1 (red) with H3K9me3 (magenta) and adjacent to centromere DNA-FISH (green) and in UOK121 (E) and UOK342 (G) cells. Insets show magnified images of boxed regions. Scale bars are 5μm. Signal intensity profiles along the lines indicated on the merged-channel images for UOK121 (F) and UOK342 (H).

(I-L) Representative immunofluorescence images and quantification illustrate the co-localization of TEAD1 (green) with TRF2 (magenta) and CENP-A markers (red) in UOK121 (I) and UOK342 (J). Insets show magnified images of boxed regions. Quantification uses 3D nearest neighbor analysis in UOK121 (K) and UOK342 (L), demonstrating a tighter distribution of CENP-A at a smaller minimal distance. Data are presented as mean ± SD.

Statistical significance determined by unpaired t-test: ns, p > 0.05; *, p ≤ 0.05; ****, p ≤ 0.0001.