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[Preprint]. 2025 May 3:2025.05.02.651992. [Version 1] doi: 10.1101/2025.05.02.651992

Figure 4. TEAD1 foci formation is dependent on DNA-binding ability.

Figure 4.

(A) Schematics of eGFP-TEAD1 constructs with wild-type TEAD1, TEAD1 DNA-binding domain (DBD) deletion, TEAD1 helix 1 (H1) mutant, and TEAD1 DBD helix 3 (H3) mutants. The underlined amino acid sequence represented the TEAD1 nuclear localization signal (NLS), and the red amino acids are mutated in the respective mutants.

(B-E) Representative images of UOK121 and UOK342 cells expressing eGFP-TEAD1-WT (B), eGFP-TEAD1-DBD deletion (C), eGFP-TEAD1-H1mut (D), and eGFP-TEAD1-H3mut (E), with immunofluorescence signal of H3K9me3 (red). Insets show magnified images of boxed regions. Scale bars are 5μm.

(F-G) Quantification of co-localization between H3K9me3 and eGFP-TEAD1 variants signals shown in (B-E). Data are presented as scatterplots with means ± SEMs.

Statistical significance determined by one-way ANOVA comparing different mutants to the TEAD1WT: ns, p > 0.05; **, p ≤ 0.002; ***, p ≤ 0.0002; ****, p ≤ 0.0001,