| Component | Final Concentration / Volume |
| 40% Acrylamide Solution (29:1 bis-acrylamide ratio) | 12% |
| 1.5 M Tris-HCl, pH 8.8 | 25% |
| diH2O | 4.4 mL |
| SDS 1 | 0.1% |
| APS 2 | 0.05% |
| TEMED 3 | 0.05% |
| 1 Prepare as a 20% stock solution and store at room temperature. 2 Prepare as a 10% stock solution, aliquot, and store at −20 °C. 3 Add immediately before pouring the gel. | |
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