| Component | Final Concentration/Volume |
| 40% Acrylamide Solution (29:1 bis-acrylamide ratio) | 4% |
| 0.5 M Tris-HCl, pH 6.8 | 25% |
| Glycerol | 25% |
| Bromophenol blue | 0.0048% |
| SDS 1 | 0.1% |
| APS 2 | 0.05% |
| TEMED 3 | 0.05% |
| 1 Prepare as a 20% stock solution and store at room temperature. 2 Prepare as a 10% stock solution, aliquot, and store at −20 °C. 3 Add immediately before pouring the gel. | |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.