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. 2025 Apr 11;482(7):BCJ20240626. doi: 10.1042/BCJ20240626

Figure 1: The PRLs promote miR-21 expression.

Figure 1:

(A) qRT-PCR analysis of miR-21 expression in HEK293 control and PRL2 overexpressing cells, normalized to RNU-6 snRNA. Each sample was analyzed in triplicate, and the Student’s t-test was used to measure significance (***P < 0.001). Western blotting showing PRL2 protein expression in PRL2-Flag or control vector transfected HEK293 cells. (B) qPCR analysis of miR-21 expression in GC-1 control and PRL2 overexpressing cells, normalized to RNU-6 snRNA. Each sample was analyzed in triplicate, and the Student’s t-test was used to measure significance (***P < 0.001). Western blotting was used to examine PRL2 expression in control and PRL2 overexpressing GC-1 cells. (C) qRT-PCR analysis of miR-21 expression in control and CRISPR-based PRL2 KO HEK293 cells, normalized to RNU-6 snRNA. Each sample was analyzed in triplicate, and the Student’s t-test was used to measure significance (***P < 0.001). Western blotting showing PRL2 protein expression in control and PRL2 KO HEK293 cells. (D) qPCR analysis of mouse miR-21 expression in control and PRL2 knockout MEF cells normalized to RNU-6 snRNA. Each sample was analyzed in triplicate, and the Student’s t-test was used to measure significance (***P < 0.001). Western blotting was used to examine PRL2 expression in control and PRL2 knockout MEF cells. (E) The mRNA level of PTP4A2 (top panel) or PTP4A3 (bottom panel) and miR-21 from Kidney renal clear cell carcinoma (KIRC, n = 587) were plotted and the Pearson rank correlation analyses were performed. Positive correlation coefficients suggest a positive correlation between PTP4A2 or PTP4A3 mRNA and miR-21 in the cancer samples (****P < 0.0001). (F) Patient samples from KIRC were divided into low-expression and high-expression groups based on the mean PTP4A2 (top panel) or PTP4A3 (bottom panel) mRNA level, and then the miR-21 level of the two groups was plotted. miR-21 level was significantly higher in the high-expression groups than the low-expression groups by Mann-Whitney tests (****P < 0.0001). (G) The mRNA level of PTP4A2 (top panel) or PTP4A3 (bottom panel) and miR-21 from brain low-grade gliomas (LGG, n = 526) were plotted and the Pearson rank correlation analyses were performed. Positive correlation coefficients suggest a positive correlation between PTP4A2 or PTP4A3 mRNA and miR-21 in the cancer samples (****P < 0.0001). (H) Patient samples from LGG were divided into low-expression and high-expression groups based on the mean PTP4A2 (top panel) or PTP4A3 (bottom panel) mRNA level, and then the miR-21 level of the two groups was plotted. The miR-21 level was significantly higher in the high-expression groups than in the low-expression groups by Mann-Whitney tests (****P < 0.0001).