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. 2025 Jun 6;125(12):5461–5583. doi: 10.1021/acs.chemrev.4c00754

5. Summary of the Yields, Conditions, IL Systems, and Feedstock Reported for the Production of 2G Ethanol with ILs.

IL biomass PT conditions saccharification and fermentation conditions yields ref
[C2C1im][(C2O)2PO2], [C2C1im]Cl, [C2C1im][C1CO2] microcrystalline cellulose Avicel 80 °C, 30 min, acetate buffer as antisolvent recombinant S. cerevisiae (with cellulase expression), 96 h, 30 °C 90% EtOH Nakashima et al. 2011
[C2C1im][C1CO2] cotton stalks 140 °C, 30 min, 30 wt% loading 10 wt% solids, 48 h, 50 °C; recombinant S. cerevisiae, 30 °C, 120 h 74.1% EtOH Haykir and Bakir, 2013
[C2C1im][C1CO2] spruce sawdust 120 °C, 15 h, H2O as antisolvent 5 wt% solids, 72 h, 45 °C; S. cerevisiae, 32 °C, 24 h 81.5% EtOH Shafiei et al. 2013 
[C2C1im][C1CO2] aspen wood 120 °C, 5 h, 10 wt% loading, H2O as antisolvent 3 wt% solids, 72 h, 30 °C; S. cerevisiae, 35 °C, 30 h 81.2% EtOH Goshadrou et al. 2013
[C2C1im][C1CO2] corn stover 140 °C, 3 h, 10 wt% loading 10 wt% solids, 72 h, 50 °C; recombinant S. cerevisiae, 30 °C, 120 h 93% EtOH Uppugundla et al. 2014
[C2C1im][C1CO2] rice straw 120 °C, 5 h, 5 wt% solids, H2O as antisolvent 10 wt% solids, 72 h, 45 °C, 20 FPU/g DM, 30 IU/ g DM; S. cerevisiae, 38 °C, 48 h 80% EtOH Poornejad et al. 2014
[Ch][C1CO2] sugarcane bagasse ultrasonication at 25 °C for 1 h, H2O as antisolvent 3.3 wt% solids, 48 h, 50 °C; S. cerevisiae, 30 °C, 24 h 75% EtOH Ninomiya et al. 2015
[C2C1im][C1CO2] Eucalyptus 150 °C, 30 min, 1:3 solid liquid-ratio, H2O as antisolvent 10 wt% solids, 72 h, 45 °C, 37 FPU/g DM, 4.9 CBU/ g DM, S. cerevisiae, 40 °C, 72 h 38% EtOH Lienqueo et al. 2016
[C2C1im][C1CO2] agave 120 °C, 3 h, 10 wt% loading 10 wt% solids, 72 h, 50 °C, 8 FPU g/DM, 15 CBU g/DM; E. coli, 30 °C, 120 h 81.6% EtOH Perez-Pimienta et al. 2017
[C2C1im][C1O(H)PO2] spruce sawdust 110 °C, 40 min, 2 wt% loading, H2O as antisolvent 10 wt% solids, 80 h, 37 °C; K. marxianus, 35 °C, 72 h 84.3% EtOH Mehmood et al. 2018
3-methylmorpholinium chloride [C1 3Morph]Cl rice straw 120 °C, 5 h, 5 wt% loading, H2O as antisolvent 5 wt% solids, 72h, 45 °C; S. cerevisiae, 30 °C, 24 h 64% EtOH Mohammadi et al. 2019
[C4C1im][C1CO2] sugarcane bagasse 120 °C, 2 h, H2O as antisolvent 10 wt% solids, 48 h, 47 °C; S. cerevisiae, 36 °C, 96 h 89.3% EtOH Smuga-Kogut et al. 2019
aqueous [C4C1im]Cl + HCl mixed softwood, hardwood, sugarcane bagasse 130 °C, 3 h 15 wt% solids, 20 wt% H2O 10 wt% solids, 72 h, 50 °C, 17.25 FPU/g DM, 6.26 CBU/ g DM, 25 FXU (xylanase); S. cerevisiae, 30 °C, 48 h 21.86–29.56 g/L EtOH, up to 99% EtOH Trinh et al. 2019
[C4C1im]Cl spruce 150 °C, 1 h, 20 wt% loading, H2O as antisolvent 5 wt% solids, 72 h, 28, 37, or 50 °C; S. cerevisiae, 37 °C, 72 h 32% EtOH Dotsenko et al. 2018
[C4C1im]Cl hornbeam 150 °C, 1 h, 20 wt% loading, H2O as antisolvent 5 wt% solids, 72 h, 28, 37 or 50 °C; S. cerevisiae, 37 °C, 72 h 36% EtOH Dotsenko et al. 2018
[C2C1im][C1CO2] oak and spruce 45 °C, 40 min, 2 wt% solids, H2O as antisolvent 80 h, 40 °C, cellulases; S. cerevisiae, 72 h, 30 °C 53–54% EtOH Alayoubi et al. 2020
[C2C1N][C1CO2] sugarcane bagasse 150 °C, 2 h, 15 wt% loading, 30 wt% H2O 10 wt% solids, 72 h, 50 °C, 15 FPU/g DM; S. passalidarum, 28 °C, 72 h 87.4% EtOH Nakasu et al. 2021
[(H2N)2C2N][C1CO2] sugarcane bagasse 140 °C, 3 h, 15 wt% loading, 30 wt% H2O 10 wt% solids, 72 h, 50 °C, 15 FPU/g DM; S. passalidarum, 28 °C, 72 h 85.3% EtOH Pin et al. 2021
[C2C2C2N][HSO4] wheat straw 130 °C, 3 h, 20 wt% solids, 20 wt% H2O, H2O as antisolvent 72 h, 50 °C, 28 FPU/g DM; S. cerevisiae, 96 h, 30 °C 84% EtOH Ziaei-Rad et al. 2021