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. 1999 Aug 15;342(Pt 1):1–6.

Increased protein phosphorylation of cytoplasmic dynein results in impaired motor function.

M T Runnegar 1, X Wei 1, S F Hamm-Alvarez 1
PMCID: PMC1220428  PMID: 10432292

Abstract

Inhibition of serine/threonine protein phosphatases in rat hepatocytes by okadaic acid and microcystin increased the phosphorylation of several components of the cytoplasmic dynein complex. UV light/vanadate cleavage and Western blot analysis revealed that two of these components with molecular masses of approx. 400 kDa and 74 kDa were dynein heavy- and intermediate-chains respectively. This increased phosphorylation resulted in inhibition of dynein ATPase activity, and reduced motor-dependent avidity of endosomal/lysosomal membranes for microtubules.

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Selected References

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