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. 2025 Jun 28;63(4):e70021. doi: 10.1002/dvg.70021

FIGURE 7.

FIGURE 7

The GnRH‐1 neurons are positive for Contactin‐2 (Cntn2) tracing. (a–e‴) Double immunostaining of GnRH‐1 (green, arrows) with Cntn2 tracing (magenta, arrowheads) and GnRH‐1 neurons that are traced for Cntn2 (white, empty arrowheads) in a time course from E12.5 (1 day post‐injection (DPI)), 2 DPI E13.5 and 4 DPI E15.5. (b) Cntn2CreERT2 +/−/Ai14+/− mice were injected with tamoxifen at E11.5 and collected at E12.5‐E15.5. The relationship between GnRH‐1 neurons (green) and GnRH‐1 neurons that are traced (white) is depicted by the pie charts next to the images. (a–a‴) E12.5 shows Cntn2 traced neurons, GnRH‐1 neurons, and Cntn2 traced neurons positive for GnRH‐1 expression within and outside of the vomeronasal organ (VNO); (c–c‴) At E13.5 there is an increase of the three populations migrating out of the VNO; Cntn2 traced neurons can be seen in the olfactory epithelium (OE) and olfactory bulb (OB). Red blood cells are marked with asterisks. (d–d‴) E15.5 shows all three populations of neurons invading the brain at the forebrain junction (FBJ), while very few are still migrating out from the VNO. Red blood cells are marked with asterisks. (e–e‴) A magnification of the basal forebrain (bFB) at E15.5, with the three populations of neurons invading. Scale bars in (c′–c‴ 25 μm; a′–a‴, d′–d‴ 50 μm; (a, c, d, e) 100 μm.