FIGURE 8.

Double injections at E11.5 and E12.5 trace GnRH‐1 neurons invading the brain. (a) Cntn2^{CreERT2 +/−}/Ai14^+/− mice were injected with tamoxifen at E11.5 and E12.5 and collected at E15.5. (b–b‴) Double immunostaining of GnRH‐1 (green, arrows) and Cntn2 tracing (magenta, arrowheads) at E15.5. Many GnRH‐1 neurons are observed to be invading the basal forebrain (bFB) with Cntn2 traced neurons and GnRH‐1 neurons that are traced for Cntn2 (white, empty arrowheads). The olfactory bulb (OB) was positive for Cntn2 tracing. Scale bars in (b, 200 μm; (b′–b‴) 100 μm.