Table 1.
CODEHOP primers used for CYP98s amplification. For each primer the consensus clamp (XXX.XXX) is given in upper case and degenerate core (xxx.xxx) in lower case. y = [C, T], n = [A, G, C, T], s = [G, C], r = [A, G]. BLASTp analysis was performed with the consensus protein sequence corresponding to each primer. Hits: best scoring P450, with expectation values lower than 0.1, are listed in the order of scores.
| Primer | Sequence | Protein consensus | BLASTp hits on P450 database (1) | Expected size of PCR product (bp) |
| Sense | ||||
| P98a | TC.ACC.GTC.GAG.tgg.gcn.atg.gc | ITVEWAMA | 98/76/93/81/79/71/705 | 270 |
| P98c | G.GTG.CAG.GAG.AAG.GTG.car.san.gar.yt | RVQEKVQEEL | 98/78 | 220 |
| P98d | G.GTG.CAG.CAG.AAG.GTC.CAG.san.gar.ytn.ga | RVQQKVQEELD | 98/78/703/96/73 | 220 |
| Reverse | ||||
| P98cr | C.CCG.GGC.GAT.Ggc.yyr.nac.rtt | NVWAIARD | 98/75/93/76/71/78/83/92/706 |
(1) BLASTp parameters: Matrix PAM40/Expectation Value (E)10.