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. 2025 Jun 24;20:8101–8118. doi: 10.2147/IJN.S516233

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© 2025 Jiang et al.

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In vitro immune escape conditions. (A) Confocal images of RAW264.7 cells cocultured with 1. PAM-SiO₂, 2. B16-PAM-SiO₂, 3. 4T1-PAM-SiO₂, 4. RAW-PAM-SiO₂, and 5. GL261-PAM-SiO₂ for 8 hours. SiO₂ content=16 µg/mL. Nuclei were stained with DAPI. Scale bar = 50 µm. (B) Flow cytometry analysis of RAW264.7 cells cocultured with B16-PAM-SiO₂ (Orange line), 4T1-PAM-SiO₂ (green line), RAW-PAM-SiO₂ (black line), GL261-PAM-SiO₂ (red line), and PAM-SiO2 (blue line) for 8 hours. (C) Average fluorescence intensity of SiO₂ in RAW264.7 cells treated with different nanoparticles. n=4. Compared with the PAM-SiO₂ group, ****p<0.0001.