. 2025 Jun 24;20:8101–8118. doi: 10.2147/IJN.S516233

Table 1.

Summary of the Key Results

Research Content	Key Methods	Summary of Results
CMCNPs Construction	• Tumor membrane extraction (centrifugation) • PAM loading (electrostatic adsorption) • Ultrasonic fusion	• Four CMCNPs were synthesized: B16-, 4T1-, RAW-, and GL261-PAM-SiO₂.
CMCNPs Characterization	• TEM imaging • Zeta potential measurements • Encapsulation efficiency tests • Release kinetic experiments	• The core–shell structure was confirmed • Zeta ↓10 mV • 80% encapsulation • The release rate was slightly lower than uncoated PAM-SiO₂
In Vitro Testing (four particles)	• hCMEC/D3 Transwell model	• 2.8× higher BBB penetration compared with uncoated NPs
In Vitro Testing (four particles)	• Immune evasion (macrophage uptake)	• 2.4× reduced phagocytosis • Ranking: GL261 > B16 > RAW > 4T1
B16-PAM-SiO₂ and GL261-PAM-SiO₂ with optimal BBB penetration and immune evasion capability were selected for in vivo testing.
In Vivo Testing (two particles)	• Fluorescence imaging • AChE activity assay • 24-h survival tracking	• 3.5× brain accumulation (B16-PAM-SiO₂) • AChE activity was high in the CNS (CMCNPs vs PAM-SiO₂ and PAM) • 100% survival (CMCNPs vs the 50% control)