Figure 7.

Quantification of muscle damage by electroporation using different voltage. The gastrocnemius muscle was injected with 50 μL (1 μg / μL) of pAdapt Empty (black bars), or 50 μL of PBS (striped bars) followed by the electric pulse delivery. The control mice received only the electric pulse delivery (white bars). The muscle and blood were collected 2 days after treatment. Four animals per group. Data are expressed as means ± SD. A: plasma levels of the Creatine kinase. B: percentage of necrotic fibers determined in the electroporated part of the muscle. Statistical analysis (Scheffe's test) showed a significant difference of the muscle damage at the highest voltage (200 and 250 V/cm) between the mice treated by electroporation without pre-injection and those pre-injected with PBS or plasmid and then treated with electroporation. There is not a significantly different damage between the muscle pre injected with PBS and the muscle pre injected with plasmid prior to the electroporation. The p values between the pre injected groups (with PBS or with plasmid) and the "electroporation only" group are indicated above the bars.