Fig. 2.

Treatment of BRCA cell lines with Diazepam and Zolpidem induced cell migration. The effects of two BZDR drugs (benzodiazepines and related Z-drugs), Zolpidem and Diazepam, on BRCA cell growth was determined by: A Trypan Blue exclusion test to study the cell growth of a non-carcinoma cell line (MCF10A), a low metastatic cell line (MCF7) and a highly metastatic cell line (MDA-MB231). Zolpidem significantly increased cell growth in MCF10A, whereas Diazepam stimulated MCF7 cell growth significantly (*p < 0.05; **p < 0.01). Image and quantitative analyses of cell migration assay were carried out for B, C MCF7 and D, E MDA-MB231 cells. Compared with DMSO control, treatment with Zolpidem and Diazepam significantly increased BRCA cell migration. The migration rate (% gap closure) was quantitatively normalized to the corresponding 0 h time point controls. Data are representative of means ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.005