Table 1.

Doubling time and vascularisation of the tumours

	N	L44 adenocarcinoma	L42 squamous cell carcinoma
Rat strain		Brown Norway	Wag/Rij
Volume Doubling time
• in vitro [hours]	4	19.3 ± 1.9	46.2 ± 5.7
• subcutaneous tumour [days]	10	3.5 ± 0.5	8 ± 0.7
• lung metastasis [days]	10	3.9 ± 0.3	9.9 ± 0.7
MVD [%] ± SD
• subcutaneous tumour	5	3.5 ± 0.1	2 ± 0.1

The in vitro doubling time is determined after two days of culture of 10⁶ cells / well in a 6-wells plate. The Volume Doubling Time of subcutaneous tumours is assessed between 1000 and 2000 mm³. For the lung metastasis the doubling time was determined between day 11 and 22 for the L44 and between day 20 and 53 for the L42 carcinoma. The micro-vascular density (MVD) represents the surface of all endothelial cells as percentage of the surface of non-necrotic tissue. This was measured in 10 × 10 × 10 mm subcutaneous tumours. The values indicate means ± SD.