Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristic | Ref. or source |
|---|---|---|
| E. coli | ||
| XL1-Blue | RecA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, lac, [F′, proAB, laclqZΔM15, Tn10 (tetr)] | 33, Stratagene |
| M15(pREP4) | Lac, ara, gal, mtl, recA+, uvr+, [pREP4, lacI, kanr] | 34 |
| Plasmids | ||
| pQE30 | High copy His-Tag expression vector | Qiagen |
| pQElytB | Expression of ispH (lytB) from E. coli | This study |
| pACYC184 | Low copy cloning vector | 35, NEB |
| pACYClytB | Expression of ispH from E. coli | This study |
| pACYClytBgcpE | Expression of ispG and ispH from E. coli | This study |
| pBluescript SKII− | High copy cloning vector | Stratagene |
| pBScyclo | Expression of xylB, ispC, ispD, ispE, and ispF from E. coli | 25 |
| pBSxispC-H | Expression of xylB, ispC, ispD, ispE, ispF, ispG, and ispH from E. coli | This study |