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Schematic restriction maps of plasmids used in this study. SgrAI canonical sites are underlined. Secondary sites are marked as SgrA′. The sequences at the respective sites are shown in parentheses. pACYC177 in B was modified at position 460 as described in Materials and Methods. In D, SapI recognition sequence is boxed and its cleavage site is shown by arrows; SgrAI/AgeI cleavage site is shown as a solid line.
