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Efficiency of secondary-site cleavage on (A) pSK7 and (B) pSK1 plasmid DNA. The locations and sequences of the SgrAI canonical and secondary sites are shown in Fig. 1 C and D. One microgram of DNA (lane 1) was digested with 50 units of SgrAI either for 1 h (lane 2) or 20 h (lane 3). Lane M, 0.1–10-kb DNA ladder.
