Figure 6.

SgrAI cleavage of the BsaHI-linearized pSK7 DNA in the absence (A) or in the presence of (B) 20 nM or (C) 200 nM of oligonucleotide duplex. The oligonucleotide sequence is shown in Fig. 5C. SgrAI reactions were carried out as described in Fig. 4. Lane 1, DNA before adding SgrAI to the reaction. Lanes 2–6: 15-μl samples were withdrawn after 1, 5, 25, 125, and 1,200 min from the start of reaction and immediately quenched by adding 7.5 μl of “stop” buffer. Lane M, 0.1–10-kb DNA ladder.