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. 2002 Jan 29;99(3):1200–1205. doi: 10.1073/pnas.032681299

Figure 1.

Figure 1

Transcriptional activities of mutant HSFs. (A) Transcriptional activities of wild-type and mutant ScHSFs are measured by levels of HSP70 mRNA by using primer extension. Primer extension products are indicated (HSP70, 309–313 nucleotides; actin, 198–200 nucleotides). Lanes 1–3, wild-type HSF; lanes 4–6, dbd-mut HSF; lanes 7–9, R274K HSF; lanes 10–12, R274G HSF. NS, non-shock, cells grown at 25°C. HS, heat shock, cells subjected to heat shock at 40°C for 30 min. R, recovery, cells put back to 25°C for 1 h after heat shock to allow recovery. (B) Western blotting analysis of wild-type and mutant HSF proteins. Yeast cell extracts were subjected to SDS/PAGE and transferred to nitrocellulose filter. The protein blot was probed with anti-FLAG antibody (Sigma) fused to the C terminus of HSFs.