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. 2002 Jan 29;99(3):1206–1211. doi: 10.1073/pnas.251674198

Figure 4.

Figure 4

Effect of GAL4-VP16 on promoter escape from nonpremelted templates. (A) DNA sequences of pG5HMC2AT+nG templates. The G residue in bold indicates the position at which transcription terminates by 3′-O-methyl GTP. WT, wild type. (B) Short transcripts in the early phase of basal and activated transcription. The positions of transcripts terminated at the G residue are indicated. Asterisks indicate the transcripts from 3 to 11 nt in length that correspond to the same transcripts indicated in Fig. 3. (C) Quantification of the short transcripts. The values are means ± SD of the relative molar amount of each transcript from three independent experiments. (D) Fold activation for each pair of basal and activated transcription. The values are means ± SD from three independent experiments.