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. 2025 Jul 1;15:21521. doi: 10.1038/s41598-025-05780-w

Table 9.

PPIX plasma and brain homogenate stability results.

Matrix Stability Bench Top Freeze–Thaw Autosampler Processed Sample Long-term
QC Level LQC HQC LQC HQC LQC HQC LQC HQC LQC HQC
Nominal Conc. (nM) 3.00 1500 3.00 1500 3.00 1500 3.00 1500 3.00 1500
Plasma Period 6 h 2 cycles 48 h 4 h 94 days
Mean Conc. (nM) 3.36 1720 3.22 1660 2.97 1480 3.14 1690 3.09 1580
Accuracy (%) 112.1 114.7 107.3 110.7 99.1 98.7 104.7 112.7 103.0 105.3
CV (%) 1.7 1.8 0.6 0.3 5.4 2.8 3.4 0.9 1.5 1.3
Brain Homogenate Period 4 h 2 cycles 72 h 4 h 98 days
Mean Conc. (nM) 3.05 1460 3.18 1630 3.01 1450 3.19 1620 3.32 1580
Accuracy (%) 101.6 97.3 106.0 108.7 100.2 96.7 106.3 108.0 110.7 105.3
CV (%) 3.3 6.7 7.7 4.4 2.0 1.6 2.6 0.7 4.4 1.3