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Closed and open configurations of ColA-ImmE2. Colicin A, blue; ImmE2 insert, green; CTE2, orange. The crystal structure of CTE9/ImmE9 (28) was used as a guide to draw ImmE2 and CTE2. Colicin A is drawn schematically to conform to the structure of colicin Ia (10), except for the C-terminal domain. The transmembrane segments between ImmE2 and H8 in the open conformation are unlabeled to indicate the dearth of information concerning their structure. (A) Trans CTE2. (B) Cis CTE2.
