Table 1.
Genome instability measured by the incidence of anaphase bridges and telomere fusion PCR products in different genetic backgrounds
| Generation
|
Anaphases
|
Fusion PCR products
|
|||
|---|---|---|---|---|---|
| Line | Total | Bridges | Ratio% | ||
| WT | G1-G4 | >400 | 0 | 0 | − |
| atm | G1-G6 | >400 | 0 | 0 | − |
| atr | G1-G4 | >400 | 0 | 0 | − |
| atm atr | G1 | 826 | 42 | 5.1 | +/− |
| tert | G4 | >400 | 0 | 0 | − |
| G5 | 385 | 1 | 0.3 | +/− | |
| atm tert | G3 | 533 | 0 | 0 | − |
| G4 | 430 | 0 | 0 | +/− | |
| G5a | 446 (314) | 97 (0) | 21.8 (0) | +/− | |
| art tert | G2 | 403 | 0 | 0 | ++ |
| G3a | 181 (518) | 137 (75) | 43.1 (12.6) | ++ | |
(+/−) Rare but persistent PCR products; (++) abundant heterogeneous products.
a
Growth defects were relatively heterogeneous among siblings, as previously observed for tert plants (Riha et al. 2001), and hence cytogenetic analysis was performed in sic k plants and their wild-type-looking siblings from the same parent (in parentheses). Values are for pools of three plants. For telomere fusion PCR data, six primer pair combinations were used.