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. 2005 Sep 15;19(18):2122–2137. doi: 10.1101/gad.1339905

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Figure 5. — Expression of the p53 isoforms in normal human tissue. (A) PCR to control primers' specificity to amplify p53 isoforms. Primers were designed to be amplified specifically by normal splicing, splice β, and splice γ and were assessed by PCR on cloned cDNA of Δ133p53, Δ133p53β, and Δ133p53γ. (B) p53 isoforms are differentially expressed in normal human tissue. p53 mRNA variants were amplified specifically by PCR from total RNA extracted from normal human tissue (Clontech). (Bn) Brain; (Ht) heart; (Ki) kidney; (Lg) lung; (Th) thymus; (Col) colon; (Bm) bone marrow; (Sp) spleen; (Pr) prostate; (Ts) testis; (Sqm) skeletal muscle; (Ut) uterus; (Fb) fetal brain; (Sc) spinal cord; (Pl) placenta; (Ag) adrenal gland; (Sg) salivary gland; (Br) breast; (Fli) fetal liver; (In) intestine; (Tg) thyroid gland; (Sto) stomach; (blk) blank. Actin is shown as a control.