Skip to main content
. 2005 Sep 15;19(18):2152–2163. doi: 10.1101/gad.346105

Figure 6.

Figure 6.

Effect of hormone administration to adult mice on tissue levels of T3 and on the target gene RC3. (A) Plasma and tissue concentrations of T4 and T3 in untreated and T3-treated wild-type and mutant mice. The data show concentrations of T4 and T3 in nanograms per deciliter, as measured by radioimmunoassay in whole plasma or tissue extracts as described in Materials and Methods. Differences between groups were analyzed by two-way ANOVA with the following results: (Plasma T4) F(3,38) = 26.877, P < 0.001; (Plasma T3) F(3,38) = 6.651, P < 0.001; (Liver T4) F(3,47) = 56.594, P < 0.001; (Liver T3) F(3,52) = 103.43, P < 0.001; (Cortex T4) F(3,25) = 54.666, P < 0.001; (Cortex T3) F(3,26) = 63.514, P < 0.001; (Cerebellum T4) F(3,27) = 50,68, P < 0.001; (Cerebellum T3) F(3,27) = 47.506, P < 0.001. (B) Expression of the thyroid hormone-regulated gene RC3 in adult wild-type and mutant mice, treated and untreated with T3. The data were quantified by measuring the optical density of autoradiograms in the caudate nucleus (CPu). Three wild-type and mutant mice, treated or not with T3, were used for quantification, and the mean values ± SD are shown as average grain density in arbitrary units. The data were analyzed by one-way ANOVA: F(3,6) = 4.94, P < 0.05. Post hoc analysis showed that there was a statistically significant difference (P < 0.05) in the treated wild-type or mutant mice compared with the untreated mice.