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OP-Cu footprinting of transpososomes formed with and without H-NS. Transferred (TS) and nontransferred strand (NTS) OP-Cu footprints are shown. The +1 position is the first base pair of the transposon, and the -1 position is the first base pair of the flanking donor DNA. Boundaries for the terminal inverted repeat (IR), IHF-binding site, and flanking donor DNA are indicated. (IHFC + H-NS) IHF complex that was supershifted by H-NS.
