Figure 7.

Analysis of transposition products formed in vivo. A Southern blot with DNA isolated from isogenic E. coli strains encoding either wild-type H-NS (four separate clones) or mutant H-NS (R12C) (three separate clones) is shown. A fragment derived from the mini-Tn10 element present in each strain was used as the probe. The diagram shows the origin of the transposition products detected on the blot. (UKIC) Unkotted inversion circle; (KIC) knotted inversion circle; (DC) deletion circle; (ETF) excised transposon fragment; (URS) unreacted substrate plasmid; (^★) transposase-encoding plasmid, which cross-hybridizes weakly with the probe.