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. 2002 Feb 5;99(3):1341–1346. doi: 10.1073/pnas.032438799

Figure 2.

Figure 2

In vivo hematopoietic activity of Sca-1 and CD45-sorted populations of muscle-derived cells. Muscle was isolated from C57BL/6-CD45.1 mice and magnetically enriched for Sca-1 or CD45 expression. Positive and negative populations were transplanted into lethally irradiated C57BL/6-CD45.2 recipients along with 2 × 105 C57BL/6-CD45.2 WBM cells. PB of recipients was analyzed via FACS for CD45.1 and hematopoietic lineage markers at the indicated time points after transplantation. In the representative Sca-1 transplant shown, Thy.1 staining was not performed at 20 weeks after transplant and therefore does not appear.