Table 1.

MMK-1 peptide desensitizes THP-1 cells to D2D3_88–274

Stimulants in lower well	Pretreatment of cells
	No addition	250 nM MMK-1	500 nM MMK-1
No addition	100  ± 3.6	102  ± 3.6	96  ± 3.3
D2D3, 1 nM	161  ± 11.1*	96  ± 4.4	99  ± 4.1
D2D3, 0.1 nM	182  ± 10.2*	108  ± 5.4	98  ± 5.9
D2D3, 0.01 nM	205  ± 12.2*	118  ± 4.9	108  ± 4.2

Chemotaxis assays were carried out in triplicate as described in Materials and Methods. The value 100% refers to the migration of cells in the absence of chemoattractants. Data points are the mean ± SEM of three experiments. Desensitization was carried out by preincubating cells with the indicated concentration of MMK-1 peptide for 20 min at 37°C; MMK-1 was also present in the upper well during the assay. 

^*, P ≤ 0,0001, Student's t test.