Table 2.

Effect of pretreatment of HEK293 FPRL1/LXA4R cells with activated uPAR on the chemotactic response to fMLP

Desensitizers	% of migration	n
None	100  ± 7	4
D2D3, 0.01 μM	57  ± 3	4
D2D3, 0.1 μM	49  ± 3	2
Peptide 3*, 10 μM	71  ± 4	3
Peptide 3, 100 μM	2  ± 0.6	3
Scrambled peptide 3, 10 μM	96  ± 5	3
Scrambled peptide 3, 100 μM	65  ± 4	3

Chemotaxis assays were carried out in triplicate as described in Materials and Methods. Cells were preincubated (60 min at 37°C) with the desensitizers indicated and then were assayed for chemotaxis. The value 100% refers to a chemotactic index of 2.0 observed in the presence of 0.2 mM fMLP in the lower well in the absence of desensitizers. The 0 value refers to a chemotactic index of 1, i.e., no stimulation. Data are the mean ± SEM of three experiments. 

^*Peptide 3 is the synthetic chemotactic peptide located between domains 1 and 2 (sequence: AVTYSRSRYLEC).