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. 2002 Jan 29;99(3):1371–1376. doi: 10.1073/pnas.022516099

Figure 2.

Figure 2

CF increases the amount of F-actin in cells. (A) Cytoskeletal actin in smlA−, Ax4, and countin− cells. After 6 h of starvation, cells were collected and lysed at the times indicated after cAMP stimulation. Triton-insoluble cytoskeletons were isolated, and a Coomassie blue-stained SDS-polyacrylamide gel from a typical experiment is shown. (B) Anti-countin antibodies decrease the level of cytoskeletal actin. Ax4 cells were starved in the presence of preimmune or immune anti-countin antibodies. At 6 h, the Triton-insoluble cytoskeletons were isolated at the times indicated after cAMP stimulation. (C) CF levels do not affect total actin levels. Shown is a Coomassie-stained gel of the 0-sec total cell lysates used for the experiments shown in A and B. The heavy band in A–C is at 42 kDa. (D) F-actin in smlA−, Ax4, and countin− cells. After 6 h of starvation, cells were fixed and stained for F-actin with phalloidin and visualized with a confocal microscope. (E) Anti-countin antibodies decrease the level of F-actin. Ax4 cells were starved in buffer containing preimmune or immune anti-countin antibodies. The cells were fixed and stained with phalloidin and visualized with conventional fluorescence microscopy. (Bars in C and D are 20 μm.)