Table 2.
Repeatability test of the real-time PCR assay
Pathogen | Concentration (copies/μL) | Intra-assay CV | Inter-assay CV | ||
---|---|---|---|---|---|
X ± SD | CV (%) | X ± SD | CV (%) | ||
TaXJS | 108 | 14.93 ± 0.03 | 0.21 | 14.87 ± 0.12 | 0.80 |
107 | 18.55 ± 0.03 | 0.18 | 18.51 ± 0.08 | 0.44 | |
106 | 22.21 ± 0.03 | 0.15 | 22.19 ± 0.05 | 0.23 | |
105 | 25.52 ± 0.10 | 0.38 | 25.49 ± 0.10 | 0.41 | |
104 | 29.01 ± 0.10 | 0.35 | 28.93 ± 0.17 | 0.59 | |
103 | 32.15 ± 0.09 | 0.29 | 32.09 ± 0.14 | 0.43 | |
TaNM1 | 108 | 14.05 ± 0.06 | 0.40 | 14.29 ± 0.25 | 1.78 |
107 | 17.50 ± 0.16 | 0.92 | 17.59 ± 0.20 | 1.16 | |
106 | 20.91 ± 0.09 | 0.42 | 21.15 ± 0.41 | 1.93 | |
105 | 25.00 ± 0.02 | 0.10 | 24.94 ± 0.11 | 0.46 | |
104 | 28.34 ± 0.05 | 0.19 | 28.43 ± 0.16 | 0.57 | |
103 | 31.64 ± 0.20 | 0.64 | 31.51 ± 0.28 | 0.90 |