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. 2025 Jul 1;18:247. doi: 10.1186/s13071-025-06884-y

Table 2.

Repeatability test of the real-time PCR assay

Pathogen Concentration (copies/μL) Intra-assay CV Inter-assay CV
X ± SD CV (%) X ± SD CV (%)
TaXJS 108 14.93 ± 0.03 0.21 14.87 ± 0.12 0.80
107 18.55 ± 0.03 0.18 18.51 ± 0.08 0.44
106 22.21 ± 0.03 0.15 22.19 ± 0.05 0.23
105 25.52 ± 0.10 0.38 25.49 ± 0.10 0.41
104 29.01 ± 0.10 0.35 28.93 ± 0.17 0.59
103 32.15 ± 0.09 0.29 32.09 ± 0.14 0.43
TaNM1 108 14.05 ± 0.06 0.40 14.29 ± 0.25 1.78
107 17.50 ± 0.16 0.92 17.59 ± 0.20 1.16
106 20.91 ± 0.09 0.42 21.15 ± 0.41 1.93
105 25.00 ± 0.02 0.10 24.94 ± 0.11 0.46
104 28.34 ± 0.05 0.19 28.43 ± 0.16 0.57
103 31.64 ± 0.20 0.64 31.51 ± 0.28 0.90