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Incision of UV-irradiated supercoiled DNA by UvrC or Cho. A mixture of supercoiled DNA of UV-irradiated pWU5 (A, 2,150 bp) and nonirradiated pNP81 (B, 4,362 bp) was incubated with the indicated proteins. The positions of the supercoiled (sc) and relaxed (rel) forms of the plasmids are shown.
