Table 1.
Bacterial strains and plasmids
| Strain/plasmid | Relevant genotype or characteristics | Source or ref. |
|---|---|---|
| E. coli strains | ||
| KMBL1001 | F− derivative of W1485 (no known mutations) | 13 |
| BW25113 | lacIq rrnBT14ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | 12 |
| CS5430 | KMBL1001, ΔuvrC∷cam | 14 |
| CS5540 | KMBL1001, Δcho∷cam | This paper |
| CS5541 | KMBL1001, Δcho(FRT) | This paper |
| CS5550 | KMBL1001, Δcho(FRT) ΔuvrC∷cam | This paper |
| RW542 | lexA51(Def) | 11 |
| CS5616 | RW542, ΔuvrC∷cam | This paper |
| CS5617 | RW542, Δcho∷cam | This paper |
| CS5618 | RW542, Δcho(FRT) | This paper |
| CS5619 | RW542, Δcho(FRT), ΔuvrC∷cam | |
| ER2566 | fhuA2[lon] ompT lacZ∷T7 gene 1 endA1 | New England Biolabs |
| Plasmids | ||
| pKD46 | λ Red recombinase, araC-ParaB, Ap | 12 |
| pKD3 | Cm(FRT) | 12 |
| pCP20 | FLP, Ap, Cm | 12 |
| pWU1 | ori pSC101, Km, Tc | 14 |
| pCA179 | pWU1 with uvrC(R42A), Tc | 14 |
| pTYB1 | PT7, intein tag, Ap | New England Biolabs |
| pCA170 | cho cloned into pTYB1 | This paper |
| pWU5 | 1,330-bp cam fragment inserted in pHSS6 | Our laboratory |
| pNP81 | 2,300-bp uvrB fragment inserted in pHSS6 | Our laboratory |