. 2002 Jan 22;99(3):1473–1478. doi: 10.1073/pnas.032488499

Table 3.

In vitro methylation of RF1 and RF2 by purified HemK or HemK_P184A,P185A

HemK (0.1 μg)/substrate (1 μg)	³H incorporation (cpm)^*
HemK (0.1 μg)/substrate (1 μg)	RF1-FLAG	6xHis-RF2
BSA^†	61 ± 20	45 ± 9
HemK (wild type)	1,701 ± 255	1,246 ± 89
HemK_P184A,P185A	79 ± 22	47 ± 6

Incorporation of ³H into TCA-insoluble material was assayed as described in Materials and Methods. The average ± standard error from at least three experiments is shown.

^†

BSA was used instead of HemK.