Figure 3.

Assessment of AHR to inhaled MCh in conscious mice. Sham-treated wild-type (○) or CCR3-deficient (●) mice were exposed to aerosolized saline, and OA-sensitized wild-type (□) or CCR3-deficient (■) mice were exposed to aerosolized OA on days 21–24. Airway responses to increasing concentrations of MCh were assessed 22–26 h after the last aerosol exposure by using barometric whole-body plethysmography. The dose–response curves of MCh-induced increases in Penh are shown. Results are expressed as the means ± SEM (n = 8–12 mice/group) of the percent increase in Penh compared with the baseline Penh values after saline exposure. Significant differences between wild-type sham-treated and wild-type sensitized/challenged mice are indicated as *, P < 0.02–P < 0.001, and significant differences between sensitized/challenged wild-type and sensitized/challenged CCR3−/− mice are indicated as **, P < 0.005, as determined by unpaired Student's t test.