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Gene targets, primers, and allele-specific probes used in this study.
| Probe type | Primer sequence (5′ → 3′) | Length (bp) | Probe sequence | Label |
|---|---|---|---|---|
| MGB probe | F-TCGCCTTGACCATGTTTGAA | 104 | FAM-ATATAAATGGAAGCCCC(A)CA-MGB | S2 vaccine strain |
| R-TGCATTCGTCTCCTCCTTATTAGC | VIC-CATATAAATGGAAGCCCC(G)CA-MGB | Other 29 strains | ||
| Affinity Plus probe | F-ACCATGTTTGAATAATATGTTTGCATATA | 88 | FAM-AAGCCCC(A)CAATC-IABkFQ | S2 vaccine strain |
| R-TCTCCTCCTTATTAGCGGAC | VIC-CCCC(G)CAATCG-IABkFQ | Other 29 strains |
