Table 1.
Summary of transplantation study with 17 C.IL-6 donor mice
| Donor mice
|
Recipient mice
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Donor mouse* | Histological† diagnosis | Tissue‡ | IgH/Myc§
|
Tumor¶ | Recip.‖ | IgH/Myc§
|
Conserved IgH/Myc** | ||
| PCR | Southern | PCR | Southern | ||||||
| 8184 | PCT | PP | — | − | PCT | 4 | — | ++ | +(S) |
| PCH | MLN | — | − | PCT | 4 | — | ++ | +(S) | |
| PCH | PLN | — | − | PCT | 4 | — | ++ | — | |
| 8191 | DLCL | PP | (1) | + | PCT | 4 | (1) | − | +(P) |
| PCH | MLN | (1)+4 | − | PCT | 4 | (1) | − | +(P) | |
| PCH | PLN | (1)+1 | − | PCT | 4 | (1) | ++ | +(P) | |
| PCH | SPL | (1) | − | PCT | 4 | (1) | ++ | +(P) | |
| 8197 | FBL | MLN | 1 | − | FBL | 4 | — | − | — |
| 8198 | FBL | PP | (1) | + | PCT | 4 | (1) | + | +(S) |
| PCH | MLN | — | + | PCT | 4 | — | + | +(S) | |
| 8235 | FBL | PLN | (2) | − | FBL + PCT‡‡ | 2 | 1 | − | — |
| 8263 | PCT | MLN | (1) | − | PCT | 2 | 1 | + | — |
| PCH | PLN | — | − | PCT | 2 | 1 | + | — | |
| PCH | SPL | — | − | PCT | 2 | 1 | + | — | |
| 8270 | FBL | MLN | 3 | − | FBL + PCT‡‡ | 2 | 1 | + | +(P) |
| PCH | PLN | — | − | PCT | 2 | — | − | — | |
| PCH | SPL | — | − | PCT | 2 | — | − | — | |
| 8293 | FBL | PLN | 3 | − | PCT | 2 | 1 | + | +(P) |
| 8294 | FBL | MLN | 2 | − | FBL + PCT‡‡ | 2 | 1 | − | — |
| 8296 | FBL | PLN | 3 | − | FBL | 2 | — | + | — |
| PCH | PP | (1) | − | PCT | 2 | — | − | — | |
| PCH | SPL | 1 | − | PCT | 2 | 1 | + | +(P) | |
| 8299 | FBL | MLN | 1 | + | PCT | 2 | 1 | ++ | +(P) |
| PCH | PLN | 4 | − | PCT | 2 | 1 | ++ | +(P) | |
Mean age of donor mice was 275 ± 75 days (range 194 to 442 days).
Histological analysis of donor tissues from which cell suspensions for in vivo transfer were prepared.
Mesenteric lymph node (MLN), peripheral lymph node (PLN), Peyer's patch (PP), and spleen (SPL).
Detection of Myc/IgH junctions by PCR and/or Southern analysis. Numbers indicate how many cell clones with distinct Myc/IgH junctions were found in the same tissue. Numbers without parenthesis: reciprocal Myc/IgH junctions indicative of der 12 and der 15 were detected. Numbers in parenthesis: only Myc/IgH junctions indicative of der 15 were detected. Southern blotting resulted not in detection of Myc rearrangements (−), detection of unique Myc rearrangements present in one tissue (+) or detection of the same Myc rearrangement in various tissues (++).
Type of B-cell tumor that arose in recipient mouse. Mean latency between cell transfer and tumor take was 128 ± 66 days (range 34 to 326 days). Genotyping for IL-6 transgene demonstrated that all tumors were of donor origin.
All recipient mice (no IL-6 transgene) were primed with pristane.
Precursor–product relationship between donor cells and recipient tumor was established by PCR analysis of clonotypic IgH/Myc rearrangements (P) or by Southern blotting (S).
Outgrowth of FBL in lymphoid tissues and PCT in pristane granulomas.