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Expression of p53-GAr chimeras in p53-negative Saos-2 cells. (A) Schematic representation of the FLAG-tagged (F) p53 chimeras containing N- or C-terminal GAr or GGr insertions. (B) Extracts of transiently transfected Saos-2 cells were examined by Western blotting with a monoclonal antibody specific for wild-type p53. Molecular weight markers are indicated.
