Table 1.
Response: Vulvaless hermaphrodities and sensory mutants
| Strain | Trial type | n | Mean (SEM)
|
|
|---|---|---|---|---|
| Reversals per crossing | Time, s | |||
| Male response to vulvaless hermaphrodite-derived cue | ||||
| him-5 | − | 29 | 1.32 (0.37) | 20.14 (3.00) |
| vul (dpy) | 30 | 6.28 (1.32) | 64.13 (12.63) | |
| + (dpy) | 30 | 6.81 (1.33) | 62.00 (10.87) | |
| + | 25 | 7.26 (1.30) | 98.12 (16.21) | |
| Male sensory mutant response to hermaphrodite-derived cue | ||||
| him-5 | − | 28 | 0.33 (0.13) | 29.71 (3.65) |
| + | 29 | 2.09 (0.71) | 68.55 (10.87) | |
| osm-5 | − | 28 | 1.69 (0.36) | 50.25 (8.52) |
| + | 29 | 2.11 (0.51) | 53.79 (11.16) | |
| him-8 | − | 29 | 2.49 (0.92) | 31.41 (5.04) |
| + | 29 | 4.90 (1.26) | 63.21 (14.58) | |
| osm-6 | − | 30 | 4.41 (0.76) | 49.50 (8.87) |
| + | 30 | 5.16 (1.06) | 49.83 (9.91) | |
| him-5 | − | 25 | 0.44 (0.09) | 31.52 (4.38) |
| + | 28 | 2.92 (0.94) | 68.39 (12.78) | |
| lov-1 | − | 26 | 0.64 (0.19) | 28.58 (5.04) |
| + | 31 | 5.44 (0.95) | 104.06 (11.71) | |
| him-5 | − | 27 | 0.60 (0.13) | 33.85 (4.62) |
| + | 28 | 4.00 (0.74) | 99.36 (13.16) | |
| pkd-2 | − | 29 | 1.07 (0.31) | 31.86 (5.04) |
| + | 28 | 3.41 (1.10) | 62.76 (10.86) | |
Individual males (n) from wild type and sensory mutants were introduced to both unconditioned (−) and conditioned (+) agar trials. Trials were compared for reversals per crossing and time in scoring regions during a 5-min interval. Statistical comparison of means (SEM) between trials in the vulvaless experiment were performed using Tukey's honestly significant difference; all three conditioned trials were found to be distinct from the unconditioned negative control trial at a significance level of 0.05. For sensory mutant trials, pairwise Mann–Whitney U tests were used for osm-5, P = 0.878 and P = 0.632 and P < 0.001 and P = 0.001; t tests (one-tailed) for osm-6, P = 0.285 and P = 0.490 and P = 0.064 and P = 0.024; Mann–Whitney U tests for lov-1, P < 0.001 and P < 0.001 and P = 0.057 and P = 0.064; and pkd-2, P = 0.009 and P = 0.069 and P < 0.001 and P < 0.001; reversals-per-crossing and time-in-scoring regions, significance levels for mutant and corresponding him-5 or him-8 control trials respectively. Because the mutant dpy-20(e1282) was necessary in constructing the vulvaless hermaphrodites source strain, in addition to wild-type control trials, dpy-20(e1282) control trials were run.